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Solution to Lesson 7. Enzyme Production Technology Process – Student Topic 10


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Chapter 45 Opening

Protease is an enzyme commonly used in many industries (food, tanning, detergent, …). This enzyme is obtained mainly from animals. However, in order to obtain a large amount of enzymes, it is necessary to use a lot of animals, which takes a long time and costs a lot of money for breeding. Is there a method that can produce large quantities of protease enzymes in a short time but still reduce production costs?

Detailed explanation:

The method of enzyme production from microbial sources can meet the economic requirements, allowing the enzyme to be obtained with high efficiency and easy purification, so a large amount of protease enzyme can be produced in a short time. and still reduce production costs.

Chapter 45 CH 1

What role do enzymes play in living organisms?

Detailed explanation:

Enzymes perform many important roles in living organisms:

– Catalyzing chemical reactions: thanks to enzymes, reactions in the body occur quickly under normal temperature and pressure conditions of the body.

Control of special chemical reactions: enzymes control specific chemical reactions and adjust the reaction rate according to the body’s metabolic conditions.

Chapter 45 CH 2

Describe the properties of enzymes. For example.

Detailed explanation:

Enzymes have strong activity: only a very small amount of enzymes can catalyze chemical reactions that take place quickly and easily.

– Highly specific: each enzyme is suitable for certain substrates, so it catalyzes only a few chemical reactions in the body.

– Coordination of activities: enzymes work in a chain-like fashion.

Intracellular localization: Enzymes can be soluble in the cytoplasm or localized in organelles.

Most enzymes are of natural origin and are non-toxic.

Enzymes are affected by many factors: in an inappropriate environment, the enzyme will lose its activity.

CH tr 46 LT

What happens to the body if the production of enzymes is disrupted?

Detailed explanation:

If the enzyme production is disturbed, the chemical reactions in the body will proceed without the enzyme’s catalysis, so the speed is very slow, increasing the cell temperature … leading to the rate of metabolism and metabolism. The body’s metabolism is also slowed down, or stalled, affecting the body’s normal functioning.

Chapter 47 CH 3

What are the steps involved in the production of enzyme technology?

Detailed explanation:

The technological process of enzyme production consists of 4 main steps:

1. Select raw materials -> 2. Enzyme extraction -> 3. Enzyme purification -> 4. Enzyme preparation.

Chapter 47 CH 4

What should be kept in mind when selecting raw materials to obtain enzymes? Why?

Detailed explanation:

When selecting raw materials to obtain enzymes, it is important to note:

Choose raw materials that contain a large amount of enzymes.

Materials that allow enzymes to be tested with high efficiency

– Ease of enzyme purification.

CH tr 48 CH 5

What are the difficulties in the extraction of enzymes from the organism? To solve that problem, what method was used?

Detailed explanation:

The extraction of enzymes from the organism is difficult and the options offered to solve the difficulties are:

CH tr 48 CH 6

Why is it necessary to use grinding aids when extracting enzymes from plant cells, yeasts and microorganisms, but not animal cells?

Detailed explanation:

The reason is due to the structure of the cell’s protective membrane:

  • In animal cells, the plasma membrane is responsible for protecting the cell, the outside of the plasma membrane has no wall, so it can be removed by cutting and processing in the assimilation device.
  • In plant cells, yeasts, microorganisms, outside the cell membrane, there is also a protective cell wall structure (in plants, it is a cellulose wall, in microorganisms it is a peptidoglycan wall, …) support of grinding aids to break down solid structures such as cell walls.

Chapter 48 CH 7

Why is lysozyme used in the separation of enzymes from bacteria?

Detailed explanation:

Because lysozyme has antibacterial activity, it is able to transform insoluble polysaccharides on the bacterial cell wall, thereby breaking the bacterial cell wall and making enzyme extraction easier.

Chapter 48 CH 8

What methods are used to remove other substances from the enzyme?

Detailed explanation:

To remove other substances from the enzyme, the enzyme purification step is carried out as follows:

– To remove salts and low molecular weight compounds: dialysis with water and dilute buffer solutions; or filter through gel or dialysis bag for removal.

– For proteins and impurities with high molecular weight: combination of selective denaturation method + fractional precipitation with neutral salts + chromatography + electrophoresis…

Chapter 49 CH 9

After receiving the enzyme preparation, what should be done to keep the enzyme activity during storage and use?

Detailed explanation:

After obtaining the enzyme preparation, it is necessary to maintain the shape of the enzyme by using additives, modifying the covalent bonds or immobilizing the enzyme to keep the enzyme activity during storage and use. .

CH tr 49 LT

What is the significance of preserving the enzyme’s spatial structure in enzyme production?

Detailed explanation:

Keeping the spatial structure of the enzyme is very important in the production of enzymes because most of the enzyme preparations after being created need packaging, transportation and supply for many different fields, so It is necessary to maintain the shape of the enzyme to ensure that the enzyme activity remains unchanged.

Chapter 49 CH 10

Study Figures 7.5, 7.6, 7.8, and 7.10 to show:

a) Each stage in the production of enzymes corresponds to which stage in Figure 7.3.

b) What is the difference between the production of enzymes from plants and the production of enzymes from microorganisms.

Detailed explanation:



Same: have all the steps of the general enzyme production process.


CH tr 50 VD

Based on the production process of bromelain enzyme in pineapple, please learn and propose the production process of papain enzyme from papaya resin.

Detailed explanation:

I propose the process to produce papain enzyme from papaya resin as follows:

Chapter 53 BT 1

Choose a learned enzyme production process and suggest ways to improve that process for greater efficiency.

Detailed explanation:

I chose the process of producing bromelain enzyme from pineapple.

Proposing a way to improve that process by filtering the pineapple puree twice, which has the effect of not leaving the filtrate from the pineapple, the second time taking the filtrate will ensure that all the juice is taken. filtrate from pineapple.

BT 2

Complete the following table about the sources, effects, and applications of some enzymes.

Detailed explanation:

Chapter 53 BT 3

Learn and present a technological process for the production of an enzyme applied in the food industry (textile, tanning, …).

Detailed explanation:

The production process of cocoa fermentation microbial products contributes to improving the use value and added value of Dong Nai cocoa fruit.

Purpose: aimed at stabilizing cocoa bean fermentation, developing cocoa liquor and fermented cocoa juice with characteristic aroma.

Production process:


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